The addition of curcumin to the diet of post-weaning dairy calves: effects on ruminal fermentation, immunological, and oxidative responses.

Incorporating Curcumin into animal diets holds significant promise for enhancing both animal health and productivity, with demonstrated positive impacts on antioxidant activity, anti-microbial responses. Therefore, this study aimed to determine whether adding Curcumin to the diet of dairy calves would influence ruminal fermentation, hematologic, immunological, oxidative, and metabolism variables. Fourteen Jersey calves were divided into a control group (GCON) and a treatment group (GTRA). The animals in the GTRA received a diet containing 65.1 mg/kg of dry matter (DM) Curcumin (74% purity) for an experimental period of 90 days. Blood samples were collected on days 0, 15, 45, and 90. Serum levels of total protein and globulins were higher in the GTRA group (P < 0.05) than the GCON group. In the GTRA group, there was a reduction in pro-inflammatory cytokines (IL-1ß and IL-6) (P < 0.05) and an increase in IL-10 (which acts on anti-inflammatory responses) (P < 0.05) when compared to the GCON. There was a significantly higher (P < 0.05) concentration of immunoglobulin A (IgA) in the serum of the GTRA than the GCON. A Treatment × Day interaction was observed for haptoglobin levels, which were higher on day 90 in animals that consumed Curcumin than the GCON (P < 0.05). The catalase and superoxide dismutase activities were significantly higher (P < 0.05) in GTRA, reducing lipid peroxidation when compared to the GCONT. Hematologic variables did not differ significantly between groups. Among the metabolic variables, only urea was higher in the GTRA group when compared to the GCON. Body weight and feed efficiency did not differ between groups (meaning the percentage of apparent digestibility of dry matter, crude protein, and acid detergent fiber (ADF) and neutral detergent fiber (NDF). There was a tendency (P = 0.09) for treatment effect and a treatment x day interaction (P = 0.05) for levels of short-chain fatty acids in rumen fluid, being lower in animals that consumed curcumin. There was a treatment vs. day interaction (P < 0.05) for the concentration of acetate in the rumen fluid (i.e., on day 45, had a reduction in acetate; on day 90, values were higher in the GTRA group when compared to the GCON). We conclude that there was no evidence in the results from this preliminary trial that Curcumin in the diet of dairy calves interfered with feed digestibility. Curcumin may have potential antioxidant, anti-inflammatory, and immune effects that may be desirable for the production system of dairy calves.

Emotion Regulation Use Varies Across Socioecological Levels of Pandemic Stress in Older Adults.

OBJECTIVES: COVID-19 escalated stress within family/neighborhood (local) and national/cultural (global) levels. However, the impact of socioecological levels of stress on pandemic emotion regulation remains largely unexplored. METHODS: Thirty older adults from the Northeast US (63-92 years) reported on pandemic stress and emotion regulation in semi-structured interviews. Responses were coded into socioecological sources of local and global stress, and associated use of cognitive emotion regulation strategies from the Cognitive Emotion Regulation Questionnaire was explored. RESULTS: Older adults experienced significant distress at global levels, and perception of lacking top-down safety governance may have exacerbated local distress of engaging in daily activities during the COVID-19 pandemic. Participants endorsed coping with local stressors via perspective-taking, acceptance, and other adaptive strategies, while global sources of stress were associated with greater use of maladaptive strategies, including other-blame and rumination. CONCLUSION: Quantitative assessments may underestimate significant older adult distress and maladaptive coping toward global stressors. Findings should be replicated with more diverse populations beyond the COVID-19 context.

Inclusion of exogenous enzymes in feedlot cattle diets: Impacts on physiology, rumen fermentation, digestibility and fatty acid profile in rumen and meat.

The objective of this study was to evaluate if the inclusion of a blend composed of exogenous enzymes (amylase, protease, cellulase, xylanase and beta glucanase) in the individual and combined form in the feedlot steers diet has benefits on the physiology, rumen fermentation, digestibility and fatty acid profile in rumen and meat. The experiment used 24 animals, divided into 4 treatments, described as: T1-CON, T2-BLEND (0.5 g mixture of enzyme), T3-AMIL (0.5 g alpha-amylase), T4-BLEND+AMIL (0.5 g enzyme blend+ 0.5 g amylase). The concentration of mineral matter was higher in the meat of cattle of T4-BLEND+AMIL. A higher proportion of monounsaturated fatty acids was observed in the T3-AMIL group when compared to the others. The percentage of polyunsaturated fatty acids was higher in the T2-BLEND and T4-BLEND+AMIL compared to the T1-CON. The combination of exogenous enzymes in the diet positively modulate nutritional biomarkers, in addition to benefits in the lipid and oxidative profile meat.

Superficial scald development in 'Granny Smith' and 'Nicoter' apples: The role of key volatile compounds when fruit are stored under dynamic controlled atmosphere.

A positive correlation of α-farnesene and its oxidation metabolites with superficial scald is commonly reported in apples stored in air or controlled atmosphere (CA) systems, where O2 levels are above the lower oxygen limit (LOL) tolerated by the fruit. Nevertheless, the LOL can be monitored by the dynamic controlled atmosphere (DCA) techniques and to provide different physiological responses. Therefore, this study aimed to evaluate key volatile metabolites from 'Granny Smith' and 'Nicoter' ('Kanzi®') apples stored under dynamic controlled atmosphere (DCA) monitored by respiratory quotient (RQ), i. e. at extremely low oxygen partial pressures (ELO) and correlate their emissions with the incidence of superficial scald (SS). The volatile compounds (VCs) were isolated by solid phase microextraction (HS-SPME) and analyzed by gas chromatography. For the first time, higher concentrations of α-farnesene and its oxidation metabolites (6-methyl-5-hepten-2-one, and 6-methyl-5-hepten-2-ol) were negatively correlated with SS in DCA-RQ. This is likely due to the higher levels of ethanol in fruit stored under this condition having an inhibitory effect on SS incidence even when α-farnesene, 6-methyl-5-hepten-2-one, and 6-methyl-5-hepten-2-ol accumulate. Additionally, SS is more correlated to internal ethylene concentration (IEC) than α-farnesene accumulation and their metabolites, even when fruit were stored under ELO, where ethylene action is reduced.

The Role of Autobiographical Resilience Memories in Emotion Regulation: An Account of Age Differences in Mnemonic and Positive Reappraisal.

A lifetime of resilience through emotionally challenging experiences may benefit older adults, lending to emotion regulation mastery with time. Yet the influence of autobiographical experiences on momentary reappraisal, the reinterpretation of negative stimuli as more positive, has never been empirically tested. This online study examined the extent to which associating life memories of resilience with novel negative scenarios enhanced reappraisal efficacy and reduced difficulty to reappraise. Younger and older adults reappraised negative images by associating reappraisals to freely selected autobiographical resilience memories, cued autobiographical resilience memories, or by finding situational silver linings without mnemonic association (control). Changes in image emotional intensity ratings revealed no difference across reappraisal conditions for younger adults, while older adults most effectively down-regulated emotional intensity using the control reappraisal strategy. Older adults found autobiographical memories more helpful for mood regulation and less difficult to implement, and identified greater similarities between novel negative scenarios and their memories than younger adults. Surprisingly, greater similarity between resilience memories and negative images was associated with lower reappraisal efficacy for both age groups. Findings demonstrate the age-equivalent benefits of utilizing reappraisals associated with past narratives of resilience and suggest a sacrifice of immediate hedonic benefit for disproportionately greater subjective benefits with age.

Higher CO2 during controlled atmosphere storage of unshelled 'Barton' pecans or carnauba wax coating: Effect on the quality after long-term storage at two temperatures.

Pecan nuts, a healthy food, have shown an increased demand for consumption. Therefore, there must be a certain level of care to avoid quality losses, which are primarily influenced by storage conditions and time. This study evaluates the effects of long-term controlled atmosphere (CA) storage with low O2 partial pressure (pO2 - 2 kPa), combined or not with high CO2 (pCO2 - 40 or 80 kPa), carnauba wax coating (CW), and ambient atmosphere (AMB; control) at 10 and 20 °C, on unshelled 'Barton' pecan nut quality. Color, water activity (Aw), moisture content (MC), and oxidation markers, such as peroxide value (PV), acidity value (AV), TBARS, and volatile compounds (VC) were evaluated. Storage up to twelve months at 10 °C and with CA (regardless of the temperature) ensured higher luminosity and color parameter b* ("golden") and a lower a* parameter ("reddish"). The MC ranged from 2.8 to 3.6%, irrespective of storage conditions, for up to twelve months, which is suitable. The AMB at 10 °C ensured lower AV, TBARS, and PV compared to the CW treatment. Furthermore, CA with low pO2 and high pCO2, even at 20 °C, guaranteed lower AV, TBARS, and PV. Storage at 20 °C increased characteristic VCs of lipid oxidation (aldehydes, acids, alcohols, ketones, lactones, and esters, especially with CW coating). However, all conditions at 10 °C and with pCO2 (even associated at 20 °C) reduced the presence of these VCs. The CW used as a coating on the pecans did not show satisfactory results and should not be recommended for pecan storage at these applied conditions. Our findings showed that low pO2 and high pCO2 maintain better pecan quality than O2 ambient (20 kPa), even at temperatures above refrigeration (20 °C). Nevertheless, there were no significant differences between 40 and 80 kPa CO2.

Effects of controlled atmosphere and storage temperature on the quality of shelled 'Barton' pecan nuts during long-term storage.

This study aimed to evaluate the effects of controlled atmosphere (CA) storage at a low oxygen partial pressure (pO2; 2 kPa of O2) and low pO2 associated with high pCO2 (2 kPa O2 + 15 kPa CO2) in relation to ambient atmospheric conditions (control), at different temperatures, on shelled 'Barton' pecan nuts quality after storage. Color, respiration rates, moisture content (MC), and oxidation markers, such as peroxide value (PV), acidity value (AV), and volatile compounds (VC), were evaluated. During six months of storage, the MC decreased in all CA treatments, and treatments at 10 °C had the lowest AVs and PVs. However, the treatment with high pCO2 levels also guaranteed lower AVs at 20 °C. The color parameter b* (yellow), which is related to the golden appearance of pecans and is a highly desirable visual attribute in the commercialization of nuts, was maintained high in treatments at 10 °C with CA treatments until six months of storage. The VCs, characteristic of lipid oxidation (aldehydes, acids, alcohols, and ketones), increase in all treatments with prolonging storage. When pCO2 was associated to 10 °C at six month of storage, it showed a larger area in the acids and unsaturated aldehyde classes, while it was relevant for alcohols, lactones, and esters at 20 °C, with sweet characteristics, including ethyl ethanoate, ethyl hexanoate, and butyrolactone. Thus, this study shows another advance in the storage techniques of shelled pecans, pointing to alternatives for reducing energy costs in the cooling chain.

Remembering past challenges to feel better today: Role of neural dedifferentiation and autobiographical integration in late-life reappraisal.

Socioemotional theories suggest that surviving a lifetime of life experiences enhances older adult emotional resilience, yet the role of past emotional challenges in current models of emotion regulation is overlooked. In this paper, we propose how integration of memories and hippocampal dedifferentiation may together benefit the reappraisal of novel stressors across the lifespan. First, we review mood benefits of generating positive narratives, and more integrated memories of adverse life events with age. Second, we review neural mechanisms of narrative integration and meaning-making. We propose a framework in which narrative integration and neural dedifferentiation of hippocampal memory representations may facilitate late-life reappraisal via shared positive meaning-making in ventromedial prefrontal cortex (vmPFC). While current evidence supporting this model is limited, we conclude by discussing future directions for testing its components in multivariate neuroimaging studies, and briefly review clinical implications of the proposed model.

Fungal growth, patulin accumulation and volatile profile in 'Fuji Mishima' apples under controlled atmosphere and dynamic controlled atmosphere.

The objective of this study was to evaluate fungal and patulin contamination, together with its correlation with the volatile compounds (VCs), in 'Fuji Mishima' apples (up to 25% decayed) under controlled atmosphere (CA) and dynamic controlled atmosphere with respiratory quotient (DCA-RQ) of 1.3 combined with different partial pressures of carbon dioxide (0.8, 1.2, 1.6 and 2.0 pCO2). Fruits were stored under the above conditions for 8 months at 0.5 °C plus 7 days shelf life at 20 °C. Toxigenic fungi and patulin accumulation were found in apples from all treatments. Penicillium expansum was the most prevalent species. For all storage conditions, patulin concentrations were above the maximum level allowed in Brazil (50  µg  kg-1) with an exception of DCA-RQ1.3 + 0.8 kPa CO2. This condition, with lower pCO2, showed the lowest patulin accumulation, below the legal limit. The CA provided the highest patulin concentration (166 µg  kg-1). It was observed that fungal growth could also contribute to changes in the volatile composition. Styrene and 3-methyl-1-butanol are considered P. expansum markers in some apple cultivars and were detected in the samples. However, it was not possible to identify volatile organic compounds (VOCs) that are biomarkers from P. expansum, because there were other fungi species present in all samples. In this study, styrene, n-decanoic acid, toluene, phenol and alpha-farnesene were the compounds that showed the most positive correlation with patulin accumulation. On the other hand, a negative correlation of patulin with acids has been shown, indicating that in treatments with a higher patulin concentration there were less acidic compounds.

Phytochemical characterization, genotoxicity, cytotoxicity, and antimicrobial activity of Gautheria procumbens essential oil.

This study investigated the chemical constituents of Gaultheria procumbens essential oil and is the first to relate cytogenotoxicity with oxidative metabolism and antimicrobial activity. Chromatographic analysis of the essential oil showed methyl salicylate (99.96%) and linalool (0.04%) as the major compounds. The essential oil showed no signs of cytogenotoxicity at different concentrations (1.82 to 58.34 mg mL-1). Furthermore, G. procumbens essential oil and methyl salicylate were used to evaluate the minimal inhibitory concentrations (MIC) and minimal microbicidal concentrations (MMC). The results showed efficacy against several microorganisms, including Aeromonas caviae, Candida albicans, and Mycobacterium fortuitum with MIC values ranging from 1.82 to 3.64 mg mL-1 and MMC values ranging from 3.64 to 12.67 mg mL-1, which were confirmed by time-kill kinetics. Based on our results, the essential oil is a promising alternative to developing future formulations to treat infections caused by microorganisms.

Utilização de métodos não farmacológicos para alívio da dor no trabalho de parto / Use of non-pharmacological pain relief methods in labor / Utilización de métodos no farmacológicos para aliviar el dolor durante el trabajo de parto

RESUMO: Objetivo: analisar a prática de realização de métodos não farmacológicos para alívio da dor durante o trabalho de parto. Método: estudo quantitativo, descritivo de corte transversal desenvolvido de dezembro de 2019 a setembro de 2020 em um Serviço de Enfermagem Materno Infantil do sul do Brasil. Foram realizados analises de 560 prontuários e coletas em banco de dados institucional. Utilizou-se o teste de Kolmogorov-Smirnov para a normalidade das variáveis e, para associações, os testes t para amostras independentes, Qui-quadrado ou exato de Fischer. Resultados: Constatou-se que 164 (29,3%) das parturientes realizaram pelo menos um tipo de método, sendo os mais utilizados a hidroterapia 137(24,5%), mudança de posição 124(22,1%) e exercícios de respiração 121(21,6%). Houve associação significativa p(<0,05) entre métodos, tipo de parto, gestação e paridade. Conclusão: Este estudo evidencia o perfil de parturientes que se beneficiam destas práticas e expõe a baixa frequência do seu uso, demonstrando uma área promissora para estudos e atividades de educação continuada.

ABSTRACT Objective: to analyze the practice of using non-pharmacological pain relief methods during labor. Method: a quantitative and descriptive cross-sectional study developed from December 2019 to September 2020 in a Maternal and Child Nursing Service from southern Brazil. A total of 560 medical charts were analyzed and data collection took place in the institutional database. The Kolmogorov-Smirnov test was used for normality of the variables and, for associations, the t test for independent samples, chi-square or Fischer's exact test. Results: It was found that 164 (29.3%) of the parturients resorted to at least one type of method, the most used being hydrotherapy with 137 (24.5%), change of position with 124 (22.1%) and breathing exercises with 121 (21.6%). There was a significant association (p<0.05) between methods, type of delivery, pregnancy and parity. Conclusion: This study highlights the profile of parturients who benefit from these practices and exposes the low frequency of their use, showing a promising area for studies and continuing education activities.

RESUMEN Objetivo: analizar la práctica de implementar métodos no farmacológicos para aliviar el dolor durante el trabajo de parto. Método: estudio cuantitativo y descriptivo de corte transversal desarrollado entre diciembre de 2019 y septiembre de 2020 en un Servicio de Enfermería Materno Infantil del sur de Brasil. Se analizaron 560 historias clínicas y los datos se recolectaron en una base de datos institucional. Se utilizó la prueba de Kolmogorov-Smirnov para la normalidad de las variables y, para las asociaciones, la prueba t para muestras independientes, la de chi-cuadrado o la prueba exacta de Fisher. Resultados: Se descubrió que 164 (29,3%) de las parturientas realizaron al menos un tipo de método, siendo los más utilizados hidroterapia con 137 (24,5%), cambio de posición con 124 (22,1%) y ejercicios respiratorios con 121 (21,6%). Se registró una asociación significativa (p<0,05) entre métodos, tipo de parto, embarazo actual y paridad. Conclusión: Este estudio pone en evidencia el perfil de las parturientas que se benefician de estas prácticas y expone la baja frecuencia de su uso, demostrando así un área promisoria para estudios y actividades de formación continua.

Key aroma compounds of Canastra cheese: HS-SPME optimization assisted by olfactometry and chemometrics.

An analytical method was developed to determine volatile compounds (VC) that contribute to the aroma of cheese from Serra da Canastra (Brazil) and evaluate them in three ripening stages (fresh, short-ripened, and ripened) via headspace solid-phase microextraction (HS-SPME) combined with gas chromatography (GC). Proximate and fatty acid compositions were determined to observe whether there would be changes during ripening. Multivariate designs were applied to optimize the extraction parameters of volatile compounds and assisted by GC olfactometry (GC-O) and chemometrics. The adopted strategy revealed that the best extraction condition requires 10 min of equilibration, 75.2 min of fiber exposure at 40 °C, and 1 g of sample. The data obtained evidenced the alteration of the abundance of volatile compounds, fatty acids, and proximate composition of Canastra cheese during ripening. The fatty acid profile of the samples was mainly composed of palmitic, oleic, and stearic acids. This dairy product is rich in volatile compounds and formed primarily by alcohols (n = 14), acids (n = 13), and esters (n = 11). Olfactometry indicated that the VCs that most affected the aroma of ripened Canastra cheese were acetic acid, isobutyric acid, butanoic acid, and ethyl hexanoate. The method developed effectively discriminated against Canastra cheeses at their different ripening stages.

Early skin-to-skin contact in a child friendly hospital: perceptions of the obstetric nurses.

OBJECTIVE: To know the nurses' perception about early skin-to-skin contact. METHODS: A qualitative and exploratory-descriptive study carried out at the Obstetric Center of a university hospital in southern Brazil. The participants were eight nurses who answered a qualitative interview in April 2019. The interviews followed a guiding script and were analyzed according to thematic content analysis: data sorting and classification and final analysis. RESULTS: Nurses know the skin-to-skin contact practice and the importance of doing it properly. In their work routine, they perceive that this contact does not happen as it should and they understand the importance of recording it. CONCLUSION: Nurses aim for skin-to-skin contact to be carried out in an early and uninterrupted manner; however, the limitations related to the health team's work processes are highlighted. The study reinforces the importance of reducing unnecessary interventions at birth.

Phytochemical analysis and evaluation of the antioxidant and antiproliferative effects of Tucumã oil nanocapsules in breast adenocarcinoma cells (MCF-7).

In this work was to develop an inedited nanocapsule with tucumã oil (Astrocaryum vulgare). The oil presents of phytosterols (squalene and ß-sitosterol), all-trans-beta-carotene, acids oleic and palmitic. Antioxidant activity showed a good performance in DPPH and ABTS assays. The nanocapsules were prepared and demonstrated in their characterization particle size (206 ± 0.69 nm). The cytogenotoxicity evaluation was performed using the MTT, dichlorofluorescein, nitric oxide and dsDNA PicoGreen® assays. Antitumor efficacy assays in MCF-7 cells demonstrated that free oil and tucumã nanocapsules had IC50 of 130 and 50 µg/mL, respectively. Thus, previous studies of toxicity are relevant, as they generate future subsidies, aiming at the potential application of nanostructures and in addition, the promising effect of NCs of tucumã oil on the antiproliferative effect in breast adenocarcinoma cells was evidenced.

The isolated or combined effects of dynamic controlled atmosphere (DCA) and 1-MCP on the chemical composition of cuticular wax and metabolism of 'Maxi Gala' apples after long-term storage.

Apples have a continuous hydrophobic layer that covers the surface of the fruit, which is called the cuticle. The effects of 1-methylcyclopropene (1-MCP) on the cuticular wax layer of apples were reported after cold storage, although the interaction between 1-MCP and dynamic controlled atmosphere (DCA) is not yet known. Therefore, this study aimed to analyze the effects of 1-MCP on the wax composition and metabolism of 'Maxi Gala' apples after storage in a controlled atmosphere (CA) and dynamic controlled atmosphere based on chlorophyll fluorescence (DCA-CF) and respiratory quotient (DCA-RQ; RQ = 1.3 and 1.5). The 1-MCP treatment effectively decreased ethylene production for CA and DCA-CF treatments, while in DCA-RQ treatments produced no effect. The average extracted cuticular wax content of 'Maxi Gala' apples was 16.65 g m-2 and no differences in storage conditions or 1-MCP application were observed. Alkanes, alcohols, fatty acids, aldehydes, and terpenoids were identified in the chemical composition of the cuticular waxes, being alkanes and fatty acids the predominant ones. Moreover, 1-MCP decreased fatty acid and 10-nonacosanol concentrations in the fruit. Fruit with the 1-MCP application and stored in DCA had lower α-farnesene concentrations. The wax compositions of the DCA-stored apples with and without 1-MCP were similar. However, 1-MCP treatment resulted in a greater mass loss in fruit stored in DCA.

Age differences in cancer-related stress, spontaneous emotion regulation, and emotional distress.

OBJECTIVES: Cancer risk increases with age, cancer-related stress is common and devastating to mental health of patients, yet little work has explored age differences in cancer-related stress. This study investigated sources of stress related to cancer diagnosis and treatment and its association with age and emotional health. Though not an a priori aim of the study, adaptive strategies mentioned within discussions of stress-which we classify as spontaneous emotion regulation (ER) - were also investigated. METHOD: Participants (N = 147, aged 27-88) recruited from the VA (98% male) with oral-digestive cancers participated in semi-structured interviews regarding sources of stress 6-months post-diagnosis (T1) and treatment-related stress at 12-months post-diagnosis (T2). Patients also reported their emotional distress at T2 via the PROMIS-29. Inductive content analysis was used to classify sources of stress and ER into semantic themes and relative frequencies. RESULTS: The greatest source of stress at diagnosis was psychological; physical symptoms were the greatest source of stress at treatment. Older adults less frequently reported psychological uncertainty, social stress, and situational stress, whereas age groups reported similar rates of physical stress. When describing stress, older adults more often made spontaneous references to emotion regulation (ER). Across age groups, those who reported stress without ER in qualitative comments had higher emotional distress on the PROMIS-29 than those reporting stress with ER or no stress. CONCLUSION: ER may be key to psychological adjustment to cancer, especially in later-life. implications for assessment of stress at pivotal visits and mental health referral are discussed.

Effects of hunger on emotional arousal responses and attention/memory biases.

In this study, we examined how emotional arousal interacts with hunger states and the processing of food stimuli. In general, arousal enhances the processing of high-priority information at the expense of lower priority information (Mather & Sutherland, 2011). Because food has been a biologically relevant stimulus in primates throughout evolution, detecting it in the environment and remembering its location has high priority. In our study, inducing arousal enhanced attention to subsequent food stimuli. In addition, we manipulated whether participants were hungry or sated to examine how hunger states would influence emotional processing. Previous research reveals that being hungry is associated with increases in norepinephrine, a key neurotransmitter involved in the arousal response. We found that, when sated, participants showed greater pupil dilation to emotional than neutral stimuli. In contrast, when hungry, pupil dilation responses were as strong to neutral as to emotional stimuli. Thus, when hungry, participants were less effective at differentiating the intensity of arousal responses to emotional versus neutral stimuli because of high arousal responses to neutral stimuli. Memory for food stimuli was enhanced compared with memory for nonfood stimuli for all participants but especially for hungry participants. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

Achyrocline flaccida essential oil from Brazil: phytochemical composition, genotoxicity, protective effects on Caenorhabditis elegans, and antimycobacterial activity.

Achyrocline flaccida (Weinm) DC. essential oil was obtained by hydrodistillation of wild flowers from southern Brazil. We explored, for the first time, the phytochemical composition, toxicity, resistance to oxidative stress in Caenorhabditis elegans, and antimycobacterial activities of A. flaccida essential oil. Twenty-four compounds were identified by GC-FID-MS and the major constituents identified were α-pinene (41.10%) and caryophyllene (30.52%). The essential oil showed no signs of genotoxicity in the comet assay and presented relevant antioxidant capacity since it prevented ROS production in the C. elegans model. Furthermore, the minimal inhibitory concentrations (MIC) results showed that M. abscessus, M. massiliense, and M. fortuitum had their growth inhibited by A. flaccida essential oil. Therefore, the essential oil of this plant is a promising alternative in the search for new compounds capable of decreasing oxidative stress and treating mycobacteriosis.

Older and younger adult definitions of emotion terms: a mixed-method content analysis.

OBJECTIVES: Conceptualizations of emotions might evolve over the course of adult development as motivations shift, but there are gaps in knowledge regarding these changes. This mixed-methods study tested theoretical predictions pertaining to age group differences in conceptualizations of emotions. METHOD: University students (N = 210, M age = 20.1 years) and community-dwelling older adults (N = 90, M age = 72.5 years) participated in three survey studies (2016-2018) conducted in person, online, or via mail and provided written narrative definitions for 11 emotion words. Responses were coded for valence, arousal, time frame, reference to self, reference to social contacts, and nature of response (i.e. example or definition). Code frequencies were compared for younger and older adults via odds ratio and logistic regression analyses. RESULTS: Younger and older adults used many of the same words in definitions of emotion terms. Older participants more often referenced situational examples in their definitions than younger participants. As expected, older adults used more low arousal language, referenced the 'self,' and included other persons more in their emotion descriptions than younger persons. Unexpectedly, younger participants used more positive language in descriptions of some positive emotions. CONCLUSIONS: Descriptions of emotion terms might serve a self-regulatory function, such as to facilitate low arousal emotion experiences for older adults or to illustrate important values, such as the greater importance of other persons to emotion experiences for older than younger adults.

Early skin-to-skin contact in a child friendly hospital: perceptions of the obstetric nurses / Contacto piel a piel temprano en un hospital amigo del niño: percepciones de las enfermeras obstetrices / Contato pele a pele precoce em um hospital amigo da criança: percepções das enfermeiras obstétricas

ABSTRACT Objective To know the nurses' perception about early skin-to-skin contact. Methods A qualitative and exploratory-descriptive study carried out at the Obstetric Center of a university hospital in southern Brazil. The participants were eight nurses who answered a qualitative interview in April 2019. The interviews followed a guiding script and were analyzed according to thematic content analysis: data sorting and classification and final analysis. Results Nurses know the skin-to-skin contact practice and the importance of doing it properly. In their work routine, they perceive that this contact does not happen as it should and they understand the importance of recording it. Conclusion Nurses aim for skin-to-skin contact to be carried out in an early and uninterrupted manner; however, the limitations related to the health team's work processes are highlighted. The study reinforces the importance of reducing unnecessary interventions at birth.

RESUMEN Objetivo Conocer la percepción de las enfermeras sobre el contacto piel a piel temprano. Método Estudio cualitativo y exploratorio-descriptivo, realizado en el Centro Obstétrico de un hospital universitario del sur de Brasil. Las participantes fueron ocho enfermeras, quienes respondieron a una entrevista cualitativa en abril de 2019. Las entrevistas siguieron un guión rector y fueron analizadas según Análisis de Contenido del tipo temático: ordenamiento y clasificación de datos y análisis final. Resultados Las enfermeras conocen la práctica del contacto piel a piel y la importancia de realizarlo adecuadamente. Se dan cuenta de que, en su rutina de trabajo, este contacto no se realiza correctamente y entienden la importancia de registrarlo. Conclusión La enfermera tiene como objetivo que el contacto piel a piel se realice de forma temprana e ininterrumpida; sin embargo, se destacan las limitaciones relacionadas con los procesos de trabajo del equipo de salud. El estudio refuerza la importancia de reducir las intervenciones innecesarias al nacer.

RESUMO Objetivo Conhecer percepções de enfermeiras sobre o contato pele a pele precoce. Método Estudo qualitativo, exploratório-descritivo, realizado no Centro Obstétrico de um hospital universitário do sul do Brasil. As participantes foram oito enfermeiras, as quais responderam a uma entrevista qualitativa em abril de 2019. As entrevistas seguiram um roteiro norteador e foram analisadas conforme Análise de Conteúdo do tipo temática: ordenação e classificação dos dados e análise final. Resultados As enfermeiras conhecem a prática do contato pele a pele e a importância de realizá-la de maneira adequada. Percebem que, em sua rotina de trabalho, este contato não é realizado adequadamente e compreendem a importância de registrar sua realização. Conclusão As enfermeiras almejam que o contato pele a pele seja realizado da maneira precoce e ininterrupta; contudo, destacam-se as limitações relacionadas aos processos de trabalho da equipe de saúde. O estudo reforça a importância da redução de intervenções desnecessárias no nascimento.